Technology in medicine has taken a new dimension. With regards to testing for presence of diseases and other anomalies, Elisa technology has taken over. The cardiac Elisa kits have been particularly so good. They are devices capable of working with the hearts of almost all animals in the world to establish any defects on it.
This process depends is an enzyme dependent process that uses color change as an indicator of reactions in reagents. The process works through an enzyme immunoassay which combines with antigens producing the subsequent color change. This test is capable of establishing the presence of both antibodies and antigens.
This exercise is capable of detecting antibodies and antigens in patients. This is very useful; it helps detect illnesses before they develop into chronic problems. Doctors are able to work on it during its early stages of development. The patient will, therefore, be able to eliminate the problems at an affordable value.
Proper working of this equipment means it is sensitive to reactions, gives accurate results, and is capable of making many detailed readings at a time. When a tool is sensitive, it can exhibit any slight change resulting from the reaction between samples and reagents. Its accuracy ensures that results obtained are free of errors, and hence, believable. They are also manufactured to work on specific problems.
Stability is also important in the working of this equipment. To ensure stability, the loss rate of the activity has to be kept as low as possible. Good storage is also very important in ensuring stability. For the purpose of minimizing the effects of the environment on this experiment, standard lab conditions are very important; room temperature, standard pressure and humidity. The temperatures within the incubator should be closely regulated. It is also important to have one person working on the experiment from beginning to end.
For proper working of this activity, the researcher must ensure that all standards, reagents and samples are prepared in advance. The next thing is addition of some samples to all the wells and incubating them for close to 2 hours. He should then aspire and add some reagents. Next, he should put it back in the incubator for an hour, and later aspire and wash the mixture three times. The substrate solution should then be added, and the mixture incubated for a period of between 20 and 25 minutes. After, this step, a stopping agent must be added to stop the reaction for the researcher to make readings.
This process applies the sandwich enzyme principle. The plate that comes with the kit is coated with antibodies that are specific to the defect to be diagnosed. Standards/samples are later put on the plate as is appropriate. The samples or standards contain biotin-conjugate antibodies that are specific for the defect. Avidin conjugate is then added to every plate before incubating.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
This process depends is an enzyme dependent process that uses color change as an indicator of reactions in reagents. The process works through an enzyme immunoassay which combines with antigens producing the subsequent color change. This test is capable of establishing the presence of both antibodies and antigens.
This exercise is capable of detecting antibodies and antigens in patients. This is very useful; it helps detect illnesses before they develop into chronic problems. Doctors are able to work on it during its early stages of development. The patient will, therefore, be able to eliminate the problems at an affordable value.
Proper working of this equipment means it is sensitive to reactions, gives accurate results, and is capable of making many detailed readings at a time. When a tool is sensitive, it can exhibit any slight change resulting from the reaction between samples and reagents. Its accuracy ensures that results obtained are free of errors, and hence, believable. They are also manufactured to work on specific problems.
Stability is also important in the working of this equipment. To ensure stability, the loss rate of the activity has to be kept as low as possible. Good storage is also very important in ensuring stability. For the purpose of minimizing the effects of the environment on this experiment, standard lab conditions are very important; room temperature, standard pressure and humidity. The temperatures within the incubator should be closely regulated. It is also important to have one person working on the experiment from beginning to end.
For proper working of this activity, the researcher must ensure that all standards, reagents and samples are prepared in advance. The next thing is addition of some samples to all the wells and incubating them for close to 2 hours. He should then aspire and add some reagents. Next, he should put it back in the incubator for an hour, and later aspire and wash the mixture three times. The substrate solution should then be added, and the mixture incubated for a period of between 20 and 25 minutes. After, this step, a stopping agent must be added to stop the reaction for the researcher to make readings.
This process applies the sandwich enzyme principle. The plate that comes with the kit is coated with antibodies that are specific to the defect to be diagnosed. Standards/samples are later put on the plate as is appropriate. The samples or standards contain biotin-conjugate antibodies that are specific for the defect. Avidin conjugate is then added to every plate before incubating.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
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