mardi 1 juillet 2014

The Best Discovery In Diagnosis; Elisa Test Kits

By Sally Delacruz


There has been invented a new method of detecting antigens and other unwanted materials in a liquid or wet sample. Researchers usually look at color change within the samples to decipher whether foreign substances exist in the sample or not. A solid-phase enzyme, immunoassay, and the Elisa test kits are the main components of this test.

The main aim for the manufacture of these tools is to foster consistency and accuracy of the results obtained. It is therefore important for them to be easy to follow, precise, sensitive and target certain types of foreign materials in the body. The kits should therefore go through serious testing with different types of samples.

The main reason for manufacturing these kits is enhancement of consistency and accuracy by way of promoting visibility. Devices used in testing, therefore, have to be easy to understand and sensitive to changes in the reagents. They also have to be specific to certain tests and must also be precise. Rigorous testing is a very important exercise in ensuring that the kits satisfy all these requirements.

These tools are very important in accelerating research. A kit that cannot deliver the biological sensitivity and reproducibility required in studies that take a long time is not good for use. It is likely to cost the buyer a lot of his resources and time. Whoever wishes to buy these kits, therefore, needs to be very cautious since many companies promise many things only to disappoint researchers.

Besides antibodies, many other factors are crucial during the manufacture of these tools. For instance, rigorous antibody screening must be done, good standards chosen, steps for sample preparation developed and essays properly validated with the help of matrices. All these process are done with the purpose of attaining effectiveness.

These instruments can detect minute organisms found in small amounts. Detecting them is vital since it helps discover many diseases before they fully develop into serious illnesses. Over time, this method has always been used in diagnosing diseases in both plants and animals. It can also be applied in fostering quality in industries.

The testing process involves attaching samples onto surfaces and later binding the antigens in samples with antibodies. Specific food substrates are then added. Color change seen in the ensuing reaction is the indicator of the absence or presence antigens.

When the kits are used in carrying out the same test, the tools on the kit are dipped into a test tube of samples collected. The set-up is then washed and then incubated in chromogens. The kit itself is made up of rods with about twelve ogives that protrude. The process of washing and incubating normally occurs when the ogive is immersed in small wells made of micro-plates containing reagents.

It is always practical to use various reagents with the equipment. Hence, detecting various antibodies and antigens is also a possibility. To do this, the Ogive has to be sensitized to the different reagents. Another way of improving the sensitivity of the devices is through increasing the amount of reagents used. The sensitivity used can be either environmental or clinical.

The fact that these facilities can be used in establishing the amount of antibodies in the human body has made it possible for the test to be used in HIV testing. Their portability is also a cause for their popularity in carrying out such tests. Nevertheless, caution should be taken in determining the best kits.




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