mardi 22 janvier 2013

The Uses For Real Time PCR Kit

By Claudine MacDonald


The real time PCR kit has been used by people who are into molecular biology for a very specific purpose. The process of polymerase chain reaction is very important in the amplification of RNA and DNA. This allows for the replication of DNA sequences in million copies used for different reasons.

The technique was developed in 1983 by Kary Mullis. PCR has since then became a very important technique for both medical research and biological laboratories. There are other applications to the technique like in DNA based phylogeny, DNA doning or genetic functional analysis. Such can also be used in the detection of diseases, genetic fingerprinting or the identification of infectious diseases.

The amplification of samples of any DNA region is done through a process that will conduct selective isolation of DNA. This is very useful in hybridization for instance. Through DNA doning though larger samples would be necessary for replication. Nonetheless, the technique of polymerase chain reaction can be done through kits available which cater to both small and large samples.

Another application of the process is with DNA sequencing. This is used to determine any unknown sequences which the amplification primers could use in Sanger sequencing. This is the isolation of any DNA sequence in order to expedite the recombinant DNA technologies. This would involve the insertion of a sequence into the plasmid or genetic material of an organism or the sample.

Getting the constructs for DNA vectors can be derived by screening the bacterial colonies through the method. This is very convenient for forensic investigations where genetic fingerprinting is a very crucial tool. This would also be a good method to use for the identification of organisms or humans.

Some of the methods are going to provide high discriminative power. With this, it will be easier to identify genetic relationships. This is done in checking the relationships of children and parents. The same process is conducted for people who want to figure out the paternity of a child.

The process could also amplify a target DNA region so that the small samples could still be used for the process. In solving crimes, the forensic experts turn to the analysis of the genetic samples for their investigation. This is ideal especially if trace DNA is the only available sample available for use.

The process would also be useful even for samples of DNA which date back from thousands of years ago. The identification of ancient animals like the mammoths was done through this process as well. As a result. The humans samples like the mummies of Egypt were subjected to it as well.

Diagnosing some diseases such as malignant lymphomas or leukemia can also use real time PCR kit. It is very effective to use the technique in cancer research especially the ones that are highly-developed. It would also help to have the conditions that will detect translocated malignant cells. It is through the kit that the conditions are identified and diagnosed. It also helps a lot to get the processes properly assessed and treated. Because of it, diseases and cases are solved and treated.




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